| 质粒名称: | pVITRO-HPV33 L1L2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52493 |
| 存储实验室: | Richard Roden |
|
载体骨架基本信息 |
|
| 载体名称: | pVITRO1-neo-mcs |
| 载体出品公司: | InvivoGen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 6295 bp |
| 完整质粒大小: | 9205 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV33 L1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1503 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BglII（在克隆过程中被销毁） |
| 3' 克隆位点: | NheI（在克隆过程中被销毁） |
| 5' 测序引物 | AAACCACCGCTAATTCAAAGC |
| 3' 测序引物 | GTGGTTTGTCCAAACTCATCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pVITRO-HPV33 L1L2 was a gift from Richard Roden (Addgene plasmid # 52493 ; http://n2t.net/addgene:52493 ; RRID:Addgene_52493) |
| 参考文献部分: | Impact of inhibitors and L2 antibodies upon the infectivity of diverse alpha and beta human papillomavirus types. Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB. PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232. eCollection 2014. 10.1371/journal.pone.0097232 PubMed 24816794 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVITRO-HPV33 L1L2 9205 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HPV33 L1 and L2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pVITRO-HPV33 L1L2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9205)
AUTHORS Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
TITLE Impact of inhibitors and L2 antibodies upon the infectivity of
diverse alpha and beta human papillomavirus types.
JOURNAL PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
eCollection 2014.
PUBMED 24816794
REFERENCE 2 (bases 1 to 9205)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9205
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(12..32)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
intron 521..1468
/label=mEF-1-alpha intron
/note="intron upstream of the start codon of mouse
EF-1-alpha"
polyA_signal 3048..3169
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3059..3078)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3113..3132
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3357..3945
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3846..3865
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 4025..4328
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
misc_feature 7186..7630
/label=FMDV IRES
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 7663..7709
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 7701..7719
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7729..8523
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7783..7802)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8393..8412
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8570..9142
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
ORIGIN
1 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
61 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
181 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
241 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
301 aggagcatgc gctcagggct gagcgcgggg agagcagagc acacaagctc atagaccctg
361 gtcgtggggg ggaggaccgg ggagctggcg cggggcaaac tgggaaagcg gtgtcgtgtg
421 ctggctccgc cctcttcccg agggtggggg agaacggtat ataagtgcgg cagtcgcctt
481 ggacgttctt tttcgcaacg ggtttgccgt cagaacgcag gtgaggggcg ggtgtggctt
541 ccgcgggccg ccgagctgga ggtcctgctc cgagcgggcc gggccccgct gtcgtcggcg
601 gggattagct gcgagcattc ccgcttcgag ttgcgggcgg cgcgggaggc agagtgcgag
661 gcctagcggc aaccccgtag cctcgcctcg tgtccggctt gaggcctagc gtggtgtccg
721 cgccgccgcc gcgtgctact ccggccgcac tctggtcttt tttttttttg ttgttgttgc
781 cctgctgcct tcgattgccg ttcagcaata ggggctaaca aagggagggt gcggggcttg
841 ctcgcccgga gcccggagag gtcatggttg gggaggaatg gagggacagg agtggcggct
901 ggggcccgcc cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgggg
961 tttttcccga agcaaccagg ctggggttag cgtgccgagg ccatgtggcc ccagcacccg
1021 gcacgatctg gcttggcggc gccgcgttgc cctgcctccc taactagggt gaggccatcc
1081 cgtccggcac cagttgcgtg cgtggaaaga tggccgctcc cgggccctgt tgcaaggagc
1141 tcaaaatgga ggacgcggca gcccggtgga gcgggcgggt gagtcaccca cacaaaggaa
1201 gagggcctgg tccctcaccg gctgctgctt cctgtgaccc cgtggtccta tcggccgcaa
1261 tagtcacctc gggcttttga gcacggctag tcgcggcggg gggaggggat gtaatggcgt
1321 tggagtttgt tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca
1381 tttttggaat ttgtcccctt gagttttgag cggagctaat tctcgggctt cttagcggtt
1441 caaaggtatc ttttaaaccc ttttttaggt gttgtgaaaa ccaccgctaa ttcaaagcaa
1501 ccggtgatat caaagatccg ccaccatgtc agtgtggaga ccttcagaag ctactgtgta
1561 cttgccacct gtaccagtga gcaaggttgt gtcaacggac gagtatgttt cccggacgtc
1621 catctattac tacgccggct cttccagact gctggcagtg ggacatccct attttagtat
1681 caaaaaccct acaaatgcca aaaagcttct cgtcccaaaa gtgagcggcc tccagtatcg
1741 agtgttcaga gtcagactcc cagaccccaa taagttcggt tttccggaca cctctttcta
1801 caatcccgac acgcagcgac tggtatgggc atgtgttgga ctggagattg gcagaggcca
1861 gcctcttggg gtgggcatca gcggacaccc gcttctgaac aagtttgatg acactgaaac
1921 cggcaacaag taccctggtc agcctggcgc tgataatcgc gagtgtctgt ctatggacta
1981 caagcagaca caactgtgtt tgctcggttg caaaccccct accggagagc actggggaaa
2041 gggtgtcgct tgtacaaacg ccgcgccagc aaacgattgc ccaccccttg agctgatcaa
2101 caccattatc gaagatgggg acatggtcga cactggcttc gggtgcatgg atttcaagac
2161 actgcaggca aataaaagcg acgttcccat agatatttgc ggatcaacct gcaagtatcc
2221 cgattatctt aaaatgacat ctgaaccgta cggcgacagt ctctttttct tcctgcggcg
2281 cgagcaaatg ttcgtgcgcc attttttcaa tcgggccgga acactcgggg aggctgtgcc
2341 tgatgatctg tacattaagg gctcaggcac caccgcctcc atccagtcca gtgcattctt
2401 tcccactcca agcgggagta tggtgacatc tgaaagccag ttgtttaata aaccatattg
2461 gctgcaaagg gcccagggcc acaataacgg gatatgctgg ggaaatcaag tatttgtgac
2521 cgtcgtcgac actacacgga gtacaaacat gaccctgtgt acccaggtca ctagcgatag
2581 tacctataag aacgaaaact ttaaggagta catccgccat gtggaggagt atgacttgca
2641 gtttgttttc cagctgtgta aagtgacgtt gacagccgaa gtgatgactt acattcacgc
2701 aatgaacccc gatatccttg aagattggca gtttggtctg acccctcccc caagcgcttc
2761 cctccaagat acgtaccggt tcgttacatc acaagccatt acttgccaga agaccgtgcc
2821 acccaaagag aaggaagacc ccttggggaa atacaccttc tgggaggtag acctcaagga
2881 gaagttcagc gcggacctgg accagttccc cctgggcagg aagtttctgc tccaggccgg
2941 gctgaaggcc aagcctaaat tgaaaagggc ggctccgacc tccactagga cttcttccgc
3001 taaaaggaag aaagtcaaga aatgatagtc tagctggcca gacatgataa gatacattga
3061 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
3121 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
3181 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
3241 aaacctctac aaatgtggta tggaaatgtt aattaactag ccatgaccaa aatcccttaa
3301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
3421 gtggtttttt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
3481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt aattaacctg
4021 caggcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
4081 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
4141 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
4201 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
4261 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
4321 ttaccatgat gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
4381 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttga ctagtggagc
4441 cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag gggagagccc agggaccgtc
4501 cctaaattct cacagaccca aatccctgta gccgccccac gacagcgcga ggagcatgcg
4561 cccagggctg agcgcgggta gatcagagca cacaagctca cagtccccgg cggtgggggg
4621 aggggcgcgc tgagcggggg ccagggagct ggcgcggggc aaactgggaa agtggtgtcg
4681 tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtatataagt gcggtagtcg
4741 ccttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg
4801 gcttccgcgg gccccggagc tggagccctg ctctgagcgg gccgggctga tatgcgagtg
4861 tcgtccgcag ggtttagctg tgagcattcc cacttcgagt ggcgggcggt gcgggggtga
4921 gagtgcgagg cctagcggca accccgtagc ctcgcctcgt gtccggcttg aggcctagcg
4981 tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg ttttttgtcc ttgctgccct
5041 cgattgcctt ccagcagcat gggctaacaa agggagggtg tggggctcac tcttaaggag
5101 cccatgaagc ttacgttgga taggaatgga agggcaggag gggcgactgg ggcccgcccg
5161 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctgtggct ttccgaagca
5221 atcgggcgtg agtttagcct acctgggcca tgtggcccta gcactgggca cggtctggcc
5281 tggcggtgcc gcgttccctt gcctcccaac aagggtgagg ccgtcccgcc cggcaccagt
5341 tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc aaggagctca aaatggagga
5401 cgcggcagcc cggtggagcg ggcgggtgag tcacccacac aaaggaagag ggccttgccc
5461 ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg ccgcatagtc acctcgggct
5521 tctcttgagc accgctcgtc gcggcggggg gaggggatct aatggcgttg gagtttgttc
5581 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt cttggaattt
5641 gcccctttga gtttggagcg aggctaattc tcaagcctct tagcggttca aaggtatttt
5701 ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt caaagcaatc cggagtatac
5761 ggatccgcca ccatgcggca caagcggagc acccggcgga agagagccag cgccacccag
5821 ctgtaccaga cctgcaaggc caccggcacc tgcccccctg acgtgatccc caaggtggag
5881 ggcagcacaa tcgccgacca gatcctgaag tacggcagcc tgggcgtgtt cttcggcggc
5941 ctgggcatcg gcacaggcag cggcagcggc ggcagaaccg gctacgtgcc catcggcacc
6001 gaccctccca ccgccgccat ccccctgcag cccattagac cccctgtgac cgtggacacc
6061 gtgggccccc tggacagcag catcgtgtcc ctgatcgagg aaaccagctt catcgaggca
6121 ggggcaccag cccccagcat ccccaccccc agcggcttcg acgtgaccac ctccgccgac
6181 accacccccg ccatcatcaa cgtgtccagc gtgggcgaga gcagcatcca gaccatcagc
6241 acccacctga accccacctt caccgagccc agcgtgctgc accctcccgc ccctgccgag
6301 gcctctggac acttcatctt cagcagcccc accgtgtcca cccagagcta cgagaacatc
6361 cccatggaca ccttcgtggt gtccaccgac agctccaacg tgaccagcag cacccccatc
6421 cccggcagca gacccgtggc ccggctgggc ctgtacagcc ggaacaccca gcaggtcaag
6481 gtggtggacc ccgccttcct gaccagcccc cacaagctga tcacctacga caaccctgcc
6541 ttcgagagct tcgaccccga ggacaccctg cagttccagc acagcgacat cagccctgcc
6601 cccgaccccg atttcctgga catcattgcc ctgcacagac ccgccatcac cagcagacgg
6661 cacaccgtgc ggttcagcag agtgggccag aaggccaccc tgaaaaccag aagcggcaag
6721 cagatcggcg ccagaatcca ctactaccag gacctgagcc ccatcgtgcc cctggaccac
6781 accgtgccca acgagcagta cgagctgcag cccctgcacg acaccagcac cagcagctac
6841 agcatcaacg acggcctgta cgacgtgtac gccgacgacg tggacaacgt gcacaccccc
6901 atgcagcaca gctactccac cttcgccacc acccggacca gcaatgtgtc catccctctg
6961 aacaccggct tcgacacccc cgtgatgagc ggccctgaca tccccagccc cctgttcccc
7021 acctcctccc ccttcgtccc tatcagccct ttcttccctt tcgataccat cgtggtggac
7081 ggcgccgact ttgtgctgca ccccagctac ttcatcctgc ggcggaggcg gaagcggttc
7141 ccctacttct tcaccgacgt gcgggtggcc gcctgatagt ctaggagcag gtttccccaa
7201 tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc caggtctaga ggggtaacac
7261 tttgtactgc gtttggctcc acgctcgatc cactggcgag tgttagtaac agcactgttg
7321 cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg gtaacaagga cccacggggc
7381 caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg actttactgc
7441 gaaacccact ttaaagtgac attgaaactg gtacccacac actggtgaca ggctaaggat
7501 gcccttcagg taccccgagg taacacgcga cactcgggat ctgagaaggg gactggggct
7561 tctataaaag cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg gaggtcggca
7621 cctttccttt gcaattactg accctatgaa tacactgact gtttgacaat taatcatcgg
7681 catagtatat cggcatagta taatacgact cactatagga gggccaccat gattgaacaa
7741 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
7801 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
7861 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
7921 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
7981 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
8041 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
8101 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
8161 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
8221 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
8281 gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
8341 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
8401 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
8461 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
8521 tgagcgggac tctggggttc gaaatgaccg accaagcgaa ttcgctagga ttatccctaa
8581 tacctgccac cccactctta atcagtggtg gaagaacggt ctcagaactg tttgtttcaa
8641 ttggccattt aagtttagta gtaaaagact ggttaatgat aacaatgcat cgtaaaacct
8701 tcagaaggaa aggagaatgt tttgtggacc actttggttt tcttttttgc gtgtggcagt
8761 tttaagttat tagtttttaa aatcagtact ttttaatgga aacaacttga ccaaaaattt
8821 gtcacagaat tttgagaccc attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt
8881 caacaccgag acatttaggt gaaagacatc taattctggt tttacgaatc tggaaacttc
8941 ttgaaaatgt aattcttgag ttaacacttc tgggtggaga atagggttgt tttcccccca
9001 cataattgga aggggaagga atatcattta aagctatggg agggttgctt tgattacaac
9061 actggagaga aatgcagcat gttgctgatt gcctgtcact aaaacaggcc aaaaactgag
9121 tccttgggtt gcatagaaag ctgcctgcag ggcctgaaat aacctctgaa agaggaactt
9181 ggttaggtac cttctgaggc ggaaa
//
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